PROTHROMBIN G20210A GENE MUTATION IN PREGNANT FEMALES WITH THROMBOTIC OBSTETRIC COMPLICATIONS

Gene Mutation With Thrombotic Obstetric Complications

Abstract

Objective: To determine the frequency of prothrombin G20210A gene mutation in pregnant females with adverse
thrombotic obstetric complication and to compare it with prothrombin G20210A gene’s frequency in control
population.
Study Design: Case control study.
Place and Duration of Study: Department of Haematology, Army Medical College Rawalpindi and Military
Hospital Rawalpindi, from Nov 2013 to Oct 2014.
Material and Methods: Sixty pregnant females were included in the study; 30 were cases with adverse thrombotic
obstetric complication, while 30 were controls. Detailed history was obtained and 3 ml blood in EDTA tube was
collected. DNA was extracted from whole blood and through RT-PCR, presence of prothrombin G20210A gene
mutation was looked for in patients and controls. Data was analyzed using SPSS 21.
Results: A total of 60 women-30 cases with thrombotic obstetric complications as ‘cases’ and 30 as ‘controls’- were
included in the study. Mean age of ‘cases’ was 28.70 ± 4.23 years while that of ‘controls’ was 27.33 ± 4.49 years.
There was no statistically significant difference among the two groups (p=0.54). In case group only one of 30
(3.3%) patients had heterozygous F2 G20210A mutation while 29 (96.7%) patients had wild type allele. In control
group, all the 30 (100%) subjects had wild type allele. The odds of finding the mutation in cases was 1:29 i.e. 0.03
as compared to zero in the control group. The difference was statistically insignificant (p= 0.5).
Conclusion: Our study shows that the frequency of F2 G20210A gene mutation in pregnant females having
adverse thrombotic obstetric complications was not significantly different from its frequency in control
population.