MOLECULAR DETECTION OF SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS-2, AN EXPERIENCE OF A TERTIARY CARE HOSPITAL MALIR

Abstract

Objective: To share our experience related to molecular detection of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) RNA in COVID-19 suspected patients reported at CMH Malir.

Study Design: Cross-sectional study.

Place and Duration of Study: Department of Microbiology, Pathology Laboratory of Combined Military Hospital Malir, from March to May 2020.

Methodology: Individuals with signs and symptoms of Coronavirus Disease-19 (COVID-19) and asymptomatic patients with history of having close contact to confirmed COVID-19 patients or travelling history were considered for SARS-CoV-2 Polymerase chain reaction (PCR) assay. Total of 1330 nasopharyngeal swabs were collected for qualitative detection of COVID-19 viral RNA by real-time reverse transcription polymerase chain reaction (RT-PCR) assay.

Results: Out of 1330 tests, 74 patients were found to be SARS-CoV 2 PCR positive. Average age of patients was 30.45 ± 31.9 years with predominance of 55 (74.3%) male patients. Within 74 patients, six (8%) died in age group ≥ 40 years. Time duration of positive PCR after initial positive PCR varied between 8 days to 45 days.

Conclusion: In this study, we noticed male predominance as they are more exposed to outside environment and susceptible to acquire the virus. Therefore, they were screened in majority. Also, we need a reliable and globally accepted test like SARS COV-2 RT-PCR for early detection of both asymptomatic and symptomatic cases. This will help us in taking appropriate steps to prevent its spread further.