@article{Asif_Mushtaq_Hassan_Akhtar_Azam_2020, title={EXPRESSION OF GATA 3 IN EPITHELIAL TUMORS}, volume={70}, url={https://www.pafmj.org/PAFMJ/article/view/3787}, abstractNote={<p><strong>Objective:</strong> To assess the expression of GATA-3 among epithelial tumors of four organs including invasive mammary carcinoma, prostatic adenocarcinoma, colorectal carcinoma and high grade serous carcinoma of ovary.</p> <p><strong>Study Design:</strong> Case series study.</p> <p><strong>Place and Duration of Study:</strong> The study was performed at Shaukat Khanum Memorial Cancer Hospital and Research Center, from Jul 2017 to Dec 2017.</p> <p><strong>Methodology:</strong> Twenty cases of each of these tumors were collected and stained with ready to use GATA-3 immunohistochemical stain (Cell marque (L50-823) mouse monoclonal antibody) using BOND III Leica auto-mated immunostainer. The expression of the antibody was assessed by two histopathologists. Diffuse nuclear staining in more than 30% of tumor cells was considered as positive.</p> <p><strong>Results:</strong> All the breast carcinomas showed diffuse strong positivity in tumor cells, while all the cases of prostatic adenocarcinoma showed negative staining. Aberrant and focal staining was observed in a few cases of high grade serous and colorectal carcinomas. Three cases of high grade serous carcinoma showed weak nuclear staining in 10% of cells. Only one case of colorectal carcinoma showed weak nuclear blush in 10 to 20% of tumor cells.</p> <p><strong> Conclusion:</strong> GATA-3 was found a sensitive and specific marker for mammary carcinomas. It is a useful diagnostic tool and should be included in a panel of immunohistochemical markers when working on a metastatic tumor of unknown origin.</p>}, number={Suppl-1}, journal={Pakistan Armed Forces Medical Journal}, author={Asif, Amna and Mushtaq, Sajid and Hassan, Usman and Akhtar, Noreen and Azam, Muhammad}, year={2020}, month={Jan.}, pages={S20–25} }