Seroprevalence of Dengue Infection in Lahore During Rainfall Season of 2024

Sidra Liaqat; Waheed uz zaman Tariq; Omar  Chughtai; Hijab Batool; Hina  Tanveer; Shehryar  Khattak

doi:10.51253/pafmj.v76iSUPPL-9.14149

Authors

Sidra Liaqat Department of Pathology, Chughtai Institute of Pathology, Lahore Pakistan
Waheed uz zaman Tariq Department of Pathology, Chughtai Institute of Pathology, Lahore Pakistan
Omar Chughtai Department of Pathology, Chughtai Institute of Pathology, Lahore Pakistan
Hijab Batool Department of Pathology, Chughtai Institute of Pathology, Lahore Pakistan
Hina Tanveer Department of Pathology, Chughtai Institute of Pathology, Lahore Pakistan
Shehryar Khattak Department of Pathology, Chughtai Institute of Pathology, Lahore Pakistan

DOI:

https://doi.org/10.51253/pafmj.v76iSUPPL-9.14149

Keywords:

Dengue Virus, Dengue Fever, IgM, IgG, ELISA, Seroprevalence

Abstract

Objective: To determine the seroprevalence of dengue virus (DENV) IgM and IgG antibodies among clinically suspected dengue cases from different population groups in Lahore during the 2024 rainfall season.

Study Design: Cross-sectional study.

Place and Duration of Study: Department of Virology, Chughtai Institute of Pathology Lahore, Pakistan from Jul to Dec 2024.

Methodology: A total of 681 clinically suspected dengue cases from various age groups were included. Serum samples were tested for anti-dengue IgM and IgG antibodies using enzyme-linked immunosorbent assay (ELISA). IgM and IgG results were analyzed independently.

Results: Of the 681 cases, 70.3% (n=479) were seropositive for dengue IgM antibodies, while 17.9% (n=122) were seropositive for dengue IgG antibodies. The highest proportion of IgG seropositivity was observed in the (25–44) year age group. September recorded the highest number of IgM-positive cases, accounting for 51.9% (n=249) of all IgM seropositive cases.

Conclusion: A substantial proportion of suspected dengue cases in Lahore showed evidence of recent and past dengue infection during the monsoon season. These findings highlight seasonal dengue transmission and support the need for continued surveillance during peak periods.

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References

1. Rathore MA, Arif M, Ghani E, Niazi SK, Hussain H. Seroprevalence of dengue virus by detection of IgG antibodies: a tertiary care hospital study in Rawalpindi. J Islam Int Med Coll 2023; 18(2): 116–120.

https://doi.org/10.57234/jiimc.june23.1644

2. Ali L, Gul Z, Ijaz A, Khalid N, Zeb F, Afzal S, et al. An overview of dengue viral infection circulating in Pakistan. J Vector Borne Dis 2022; 59(2): 109–114.

https://doi.org/10.4103/0972-9062.331412

3. Tabassum S, Naeem A, Nazir A, Naeem F, Gill S, Tabassum S. Year-round dengue fever in Pakistan, highlighting the surge amidst ongoing flood havoc and the COVID-19 pandemic: a comprehensive review. Ann Med Surg 2023; 85(4): 908–912.

https://doi.org/10.1097/MS9.0000000000000418

4. World Health Organization. Dengue hemorrhagic fever: diagnosis, treatment, prevention and control. 2nd ed. Geneva: WHO; 1997. [Internet]. [cited on 25th March 2025]. Available from: http://apps.who.int/iris/bitstream/10665/41988/1/9241545003_eng.pdf

5. Aniakwaa-Bonsu E, Amoako-Sakyi D, Dankwa K, Prah JK, Nuvor SV. Seroprevalence of dengue viral infection among adults attending the University of Cape Coast Hospital. Adv Infect Dis 2021; 11(1): 60–68.

https://doi.org/10.4236/aid.2021.111008

6. Jamal MK, Sanaei B, Naderi M, Past V, Abadi SH, Khazaei R, et al. Investigating the recent outbreak of dengue fever in Iran: a systematic review. Egypt J Intern Med 2025; 37(1): 37.

https://doi.org/10.1186/s43162-025-00411-2

7. Rahman KM, Sharker Y, Rumi RA, Khan MU, Shomik MS, Rahman MW, et al. Association between rainy days and clinical dengue fever in Dhaka, Bangladesh: findings from a hospital-based study. Int J Environ Res Public Health 2020; 17(24): 9506. https://doi.org/10.3390/ijerph17249506

8. Rehman W, Nasar-u-Minallah M, Butt I. Spatial mapping of dengue fever prevalence and its association with geo-climatic factors in Lahore, Pakistan. Environ Monit Assess 2024; 196(9): 812. https://doi.org/10.1007/s10661-024-12967-7

9. Munjal V, Chugh TD, Bansal R. Seroprevalence of dengue virus IgG and associated risk factors in India. J Infect Dev Ctries 2022; 16(8): 1374–1380. https://doi.org/10.3855/jidc.16640

10. Shrestha DB, Budhathoki P, Gurung B, Subedi S, Aryal S, Basukala A, et al. Epidemiology of dengue in SAARC territory: a systematic review and meta-analysis. Parasit Vectors 2022; 15(1): 389. https://doi.org/10.1186/s13071-022-05409-1

11. Asghar F, Hussain N, Komal F, Fatima Q. Sero-prevalence of dengue IgM antibodies in patients suspected of having dengue fever. Annu Res Rev Biol 2016; 11(4): 1–10.

https://doi.org/10.9734/ARRB/2016/30090

12. Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, et al. The global distribution and burden of dengue. Nature 2013; 496(7446): 504–507.

https://doi.org/10.1038/nature12060

13. Biswal S, Reynales H, Saez-Llorens X, Lopez P, Borja-Tabora C, Kosalaraksa P, et Efficacy of a tetravalent dengue vaccine in healthy children and adolescents. N Engl J Med 2023; 388(13): 1223–1233. https://doi.org/10.1056/NEJMoa2208234

14. Raza FA, Ashraf S, Hasnain S, Ahmad J, Iqbal M. Dengue seroprevalence and its socioeconomic determinants in Faisalabad, Pakistan: a cross-sectional study. Rev Soc Bras Med Trop 2018; 51(4): 503–507.

https://doi.org/10.1590/0037-8682-0061-2018

15. Mahmood K, Rashid M, Ansari SK, Kanani F, Iftner T. Clinical characteristics of dengue virus infections in Karachi from 2019 to 2023: a cross-sectional study. Sci Rep 2024; 14(1): 31910.

https://doi.org/10.1038/s41598-024-83425-0

16. Sahar G, Mobeen H, Ghafoor MA, Baghdadi MH, Fatima A. Seroepidemiology of Dengue Fever and the Associated Sociodemographic, Clinical, and Environmental Factors in Lahore, Pakistan. J Pharm Biomed 2025; 3(1): 83-89.

https://doi.org/10.39401/jpbm.003.01.0040

17. Zameer S, Soomro S, Baig S, Rubeen R, Sharafat S. Seasonal variation and seroprevalence of dengue fever in Karachi, Pakistan. J Muhammad Med Coll 2017; 8(1): 12–15.

18. Azami NA, Moi ML, Salleh SA, Neoh HM, Kamaruddin MA, Jalal NA, et al. Dengue epidemic in Malaysia: urban versus rural comparison of dengue immunoglobulin G seroprevalence among Malaysian adults aged 35–74 years. Trans R Soc Trop Med Hyg 2020; 114(11): 798–811.

https://doi.org/10.1093/trstmh/traa056

19. Boamah I, Odoom A, Sagoe KW, Donkor ES. Seroprevalence and serotypes of dengue virus infection in Ghana: a systematic review and meta-analysis. Diseases 2025; 13(4): 114.

https://doi.org/10.3390/diseases13040114

20. Velandia-Romero ML, Coronel-Ruiz C, Castro-Bonilla L, Camacho-Ortega S, Calderón-Peláez MA, Castellanos A, et al. Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areas. Int J Infect Dis 2020; 91(1): 9–16.

https://doi.org/10.1016/j.ijid.2019.10.045