Diagnostic Utility of Beta-Catenin in Poorly Differentiated Colorectal Carcinoma

Farukh Mir Muhammad; Farhan Akhtar; Nighat Jamal; Rabia Ahmed; Muhammad Asif; Fatima Wahan

doi:10.51253/pafmj.v73i6.6428

Authors

Farukh Mir Muhammad Department of Pathology, Pakistan Navy Ship Shifa, Karachi Pakistan
Farhan Akhtar Department of Pathology, Pak Emirates Military Hospital/ National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Nighat Jamal Department of Pathology, Pakistan Navy Ship Shifa, Karachi Pakistan
Rabia Ahmed Department of Pathology, Pakistan Navy Ship Shifa, Karachi Pakistan
Muhammad Asif Department of Pathology, Armed Forces Institute of Pathology/ National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Fatima Wahan Department of Pathology, Pakistan Navy Ship Shifa, Karachi Pakistan

DOI:

https://doi.org/10.51253/pafmj.v73i6.6428

Keywords:

Beta-catenin, Colorectal carcinoma, Immunohistochemistry

Abstract

Objective: To determine the diagnostic ability of Beta-catenin in poorly differentiated colorectal carcinomas.

Study Design: Cross-sectional study

Place and Duration of Study: Department of Histopathology PNS Shifa Hospital, Karachi Pakistan from Jun 2019 to Jun 2020

Methodology: After ethical approval from the Institutional Review Board, 60 patients of both gender and all age group,
diagnosed with a case of colorectal carcinoma on biopsy and resection specimens analyzed on histopathology on H & E
staining at PNS SHIFA were included in the study. The resection specimens of CRC included those obtained from
hemicolectomy, abdominoperineal resection and biopsies. Beta-catenin was interpreted using immunohistochemistry as
cytoplasmic and nuclear staining with varying intensity. The scoring method was 0 as negative, 1+ as weakly positive, 2+ as
moderate positive, and 3+ as strongly positive.

Results: Among the 60 patients with resection specimens included from hemicolectomy, abdominoperineal resection and
colorectal biopsies, strong nuclear positive results were observed in 30(50 %) patients, moderate nuclear positivity in 12 (20 %), weak nuclear positivity in 6(10 %) and positive cytoplasmic staining in 12(20 %) patients.

Conclusion: Beta-catenin can be used successfully as a diagnostic utility in poorly differentiated colorectal carcinoma patients.

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