Detection of Bacterial Growth in Peroperative Aqueous Humour Samples in Phacoemulsification Surgery with Reusable Versus Disposable Irrigation  Aspiration Tubing System

Irshad  Hussain; Shahid Hamid  Mahmud; Mariam  Sarwar; Muhammad Aamir  Arain; Taimoor Ashraf Khan; Rabbia  Qammer

doi:10.51253/pafmj.v75iSUPPL-4.12375

Authors

Irshad Hussain Department of Ophthalmology, Combined Military Hospital, Peshawar/National University of Medical Sciences (NUMS) Pakistan
Shahid Hamid Mahmud Department of Ophthalmology, Combined Military Hospital, Peshawar/National University of Medical Sciences (NUMS) Pakistan
Mariam Sarwar Department of Microbiology, Combined Military Hospital, Peshawar/National University of Medical Sciences (NUMS) Pakistan
Muhammad Aamir Arain Department of Ophthalmology, Combined Military Hospital, Peshawar/National University of Medical Sciences (NUMS) Pakistan
Taimoor Ashraf Khan Department of Ophthalmology, Combined Military Hospital, Peshawar/National University of Medical Sciences (NUMS) Pakistan
Rabbia Qammer Department of Ophthalmology, Combined Military Hospital, Peshawar/National University of Medical Sciences (NUMS) Pakistan

DOI:

https://doi.org/10.51253/pafmj.v75iSUPPL-4.12375

Keywords:

Aqueous humor, Bacterial culture, Culture and Sensitivity, Disposable, Infection, Irrigation aspiration tubing, Phacoemulsification.

Abstract

Objective: To detect the bacterial growth in per-operative aqueous samples during phacoemulsification surgery with reusable versus disposable irrigation aspiration tubing system.

Study Design: Quasi-experimental study.

Place and Duration of study: Eye Department, Combined Military Hospital, Peshawar Pakistan, from Jan 2023-Apr 2024.

Methodology: It was a prospective quasi-experimental study. Total of 274 patients fulfilling the inclusion and exclusion criteria were selected and divided into two groups, 137 in disposable group and 137 in autoclavable group. Pre-operative conjunctival sac sampling via a swab was done in all the patients and was sent for culture for bacterial growth. All patients underwent uneventful phacoemulsification surgery with standard preoperative cleaning and preparation of eye with 5% pyodine solution. Aqueous humor samples were taken for culture and sensitivity before intracameral injection of antibiotics.

Results: Initially 300 patients were selected and 26 were excluded based on strict inclusion & exclusion criteria. Out of 274 included patients, none of the aqueous humor samples in both groups showed any bacterial growth after the specified time. One (0.70%) patient in group A yielded bacterial growth on pre-operative conjunctival sac sampling while 3 (2.10%) patients in group B yielded growth on pre-operative conjunctival sac sampling.

Conclusion: Both reusable and disposable irrigation aspiration systems are equally effective in terms of their safety for bacterial growth.

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